How To Perform DNA Extraction Using Phenol Chloroform Method 🧑🏻‍🔬🧬👇
10:09
YouTubeBiotech Made Easy
How To Perform DNA Extraction Using Phenol Chloroform Method 🧑🏻‍🔬🧬👇
Genomic DNA (gDNA) extraction using phenol/chloroform utilize detergents such as sodium dodecyl sulfate (SDS) or cetyltrimethylammonium bromide (CTAB) for cell lysis. After cell lysis, phenol/chloroform/isoamyl alcohol (25:24:1) is added to separate lipids and other proteins into the organic phase, and the gDNA into the aqueous phase. After ...
5.3K viewsJan 9, 2022
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